I am a microscope builder and hardware developer within the Spatial Technology Platform at the Broad Institute of MIT and Harvard, specializing in method development for spatial trancriptomics and 3D fluorescence microscopy with a focus on applications for psychiatric diseases.
During my Ph.D. and postdoctoral research at the University of Washington in the Molecular Biophotonics Laboratory, I developed the first multi-resolution open-top light-sheet microscope [1,2], enabled by novel optics that we have patented and implemented worldwide [3-5]. We have demonstrated the potential for this device and 3D imaging technique to improve breast cancer staging accuracy and patient management after surgery [6]. To improve clinical translation of this method, we additionally developed artificial intelligence approaches to assist pathologists with diagnosing esophageal cancer and dysplasia using 3D pathology [7,8]. My latest conference poster may be viewed here.
More recently, my latest work at the Allen Institute for Neural Dynamics focused on developing axially swept light-sheet microscopy systems for imaging RNA assays and protein analytes in cleared tissues in situ, as well as mechanical and optical designs for expansion-assisted selective plane illumination microscopy [9].
[1] L.A. Barner, A.K. Glaser, H.Huang, L.D. True, and J.T.C. Liu, “Multi-resolution open-top light-sheet microscopy to enable efficient 3D pathology workflows,” Biomedical Optics Express 11,6605 (2020). [Link][PDF]
[2] K.W. Bishop, L.A. Erion Barner,, Q. Huan et al., "An end-to-end workflow for non-destructive 3D pathology," Nature Protocols [Link].
[3] L.A. Barner, A.K. Glaser, L.D. True, N.P. Reder, and J.T.C. Liu, ”Solid immersion meniscus lens (SIMlens) for open-top light-sheet microscopy,” Optics Letters 44,4451 (2019). [Link] [PDF]
[4] J.T.C. Liu, L.A. Barner, A.K. Glaser, “Apparatuses, systems and methods for solid immersion meniscus lenses,” WO2020150239A1 (USA), EP3911977A4 (EP), JP2022517107 (JP), CN113892042B (CN) (2019).
[5] A.K. Glaser, K.W. Bishop, L.A. Barner, et al. “A hybrid open-top light-sheet microscope for multi-scale imaging of cleared tissues,” Nature Methods 19, 613 (2022).
[6] L.A. Barner, A.K. Glaser, C. Mao, E.A. Susaki, J.C. Vaughan, S.M. Dintzis, and J.T.C. Liu, “Multi-resolution non-destructive 3D pathology of whole lymph nodes for breast cancer staging,” Journal of Biomedical Optics 27, 036501 (2022). [Link]
[7] D.M. Reddi, L.A. Barner,, W. Burke, G. Gao, W.M. Grady, and J.T.C. Liu, "Non-destructive 3D pathology image atlas of Barrett esophagus with open-top light-sheet microscopy," Arch. Pathol. Lab. Med (2023). [Link]
[8] L.A.E. Barner*, G. Gao*, D.M. Reddi, L. Lan, W. Burke, W.M. Grady, and J.T.C. Liu, “Enhanced detection of neoplasia in esophageal biopsies via non-destructive 3D pathology with deep learning triage” (submitted). *equal contribution
[9] A.K. Glaser, J. Chandrashekar, J. Vasquez, C. Arshadi, N. Ouellette, X. Jiang, J. Baka, G. Kovacs, M. Woodard, S. Seshamani, K. Cao, N. Clack, A. Recknagel, A. Grim, P. Balaram, E. Turschak, A. Liddell, J. Rohde, A. Hellevik, K. Takasaki, L.A. Erion Barner, M. Logsdon, C. Chronopoulos, S. de Vries, J. Ting, S. Perlmutter, B. Kalmbach, N. Dembrow, R.C. Reid, D. Feng, and F. Svoboda, “Expansion-assisted selective plane illumination microscopy for nanoscale imaging of centimeter-scale tissues,” bioRxiv (2023).
During my Ph.D. and postdoctoral research at the University of Washington in the Molecular Biophotonics Laboratory, I developed the first multi-resolution open-top light-sheet microscope [1,2], enabled by novel optics that we have patented and implemented worldwide [3-5]. We have demonstrated the potential for this device and 3D imaging technique to improve breast cancer staging accuracy and patient management after surgery [6]. To improve clinical translation of this method, we additionally developed artificial intelligence approaches to assist pathologists with diagnosing esophageal cancer and dysplasia using 3D pathology [7,8]. My latest conference poster may be viewed here.
More recently, my latest work at the Allen Institute for Neural Dynamics focused on developing axially swept light-sheet microscopy systems for imaging RNA assays and protein analytes in cleared tissues in situ, as well as mechanical and optical designs for expansion-assisted selective plane illumination microscopy [9].
[1] L.A. Barner, A.K. Glaser, H.Huang, L.D. True, and J.T.C. Liu, “Multi-resolution open-top light-sheet microscopy to enable efficient 3D pathology workflows,” Biomedical Optics Express 11,6605 (2020). [Link][PDF]
[2] K.W. Bishop, L.A. Erion Barner,, Q. Huan et al., "An end-to-end workflow for non-destructive 3D pathology," Nature Protocols [Link].
[3] L.A. Barner, A.K. Glaser, L.D. True, N.P. Reder, and J.T.C. Liu, ”Solid immersion meniscus lens (SIMlens) for open-top light-sheet microscopy,” Optics Letters 44,4451 (2019). [Link] [PDF]
[4] J.T.C. Liu, L.A. Barner, A.K. Glaser, “Apparatuses, systems and methods for solid immersion meniscus lenses,” WO2020150239A1 (USA), EP3911977A4 (EP), JP2022517107 (JP), CN113892042B (CN) (2019).
[5] A.K. Glaser, K.W. Bishop, L.A. Barner, et al. “A hybrid open-top light-sheet microscope for multi-scale imaging of cleared tissues,” Nature Methods 19, 613 (2022).
[6] L.A. Barner, A.K. Glaser, C. Mao, E.A. Susaki, J.C. Vaughan, S.M. Dintzis, and J.T.C. Liu, “Multi-resolution non-destructive 3D pathology of whole lymph nodes for breast cancer staging,” Journal of Biomedical Optics 27, 036501 (2022). [Link]
[7] D.M. Reddi, L.A. Barner,, W. Burke, G. Gao, W.M. Grady, and J.T.C. Liu, "Non-destructive 3D pathology image atlas of Barrett esophagus with open-top light-sheet microscopy," Arch. Pathol. Lab. Med (2023). [Link]
[8] L.A.E. Barner*, G. Gao*, D.M. Reddi, L. Lan, W. Burke, W.M. Grady, and J.T.C. Liu, “Enhanced detection of neoplasia in esophageal biopsies via non-destructive 3D pathology with deep learning triage” (submitted). *equal contribution
[9] A.K. Glaser, J. Chandrashekar, J. Vasquez, C. Arshadi, N. Ouellette, X. Jiang, J. Baka, G. Kovacs, M. Woodard, S. Seshamani, K. Cao, N. Clack, A. Recknagel, A. Grim, P. Balaram, E. Turschak, A. Liddell, J. Rohde, A. Hellevik, K. Takasaki, L.A. Erion Barner, M. Logsdon, C. Chronopoulos, S. de Vries, J. Ting, S. Perlmutter, B. Kalmbach, N. Dembrow, R.C. Reid, D. Feng, and F. Svoboda, “Expansion-assisted selective plane illumination microscopy for nanoscale imaging of centimeter-scale tissues,” bioRxiv (2023).